2FB0
Crystal Structure of Conserved Protein of Unknown Function from Bacteroides thetaiotaomicron VPI-5482 at 2.10 A Resolution, Possible Oxidoreductase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-04 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 41 3 2 |
| Unit cell lengths | 118.360, 118.360, 118.360 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.100 |
| R-factor | 0.19864 |
| Rwork | 0.197 |
| R-free | 0.22440 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.120 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.157 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.110 | |
| Number of reflections | 17077 | |
| <I/σ(I)> | 36.23 | |
| Completeness [%] | 100.0 | 99.92 |
| Redundancy | 24.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 291 | 2M Ammonium Acetate, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
