2FAZ
Ubiquitin-Like Domain of Human Nuclear Zinc Finger Protein NP95
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-30 |
| Detector | SBC-3 |
| Wavelength(s) | 0.97868 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 89.554, 89.554, 108.279 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 22.800 - 2.000 |
| R-factor | 0.21959 |
| Rwork | 0.218 |
| R-free | 0.25243 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1sif |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.736 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.800 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.056 | 0.626 |
| Number of reflections | 17642 | |
| <I/σ(I)> | 28.67 | 2.36 |
| Completeness [%] | 98.5 | 99 |
| Redundancy | 5.1 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | Well Solution: 20.0% P3350, 0.2 M tri-Li Citrate; Protein Buffer: 20 mM Tris pH 8.0, 100 mM NaCl, 5 mM beta- mercaptoethanol, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
