2FAT
An anti-urokinase plasminogen activator receptor (UPAR) antibody: Crystal structure and binding epitope
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 90 |
Detector technology | CCD |
Collection date | 2004-10-05 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 37.162, 84.474, 134.030 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.290 - 1.770 |
R-factor | 0.239 |
Rwork | 0.239 |
R-free | 0.27300 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 2.100 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.290 | 1.810 |
High resolution limit [Å] | 1.770 | 1.770 |
Rmerge | 0.069 | 0.512 |
Number of reflections | 41141 | |
<I/σ(I)> | 24 | 2 |
Completeness [%] | 94.8 | 58.4 |
Redundancy | 6.5 | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 28%(w/v) PEG 3350, 100mM Tris-HCl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |