2FAI
Human Estrogen Receptor Alpha Ligand-Binding Domain In Complex With OBCP-2M and A Glucocorticoid Receptor Interacting Protein 1 NR Box II Peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-BM-C |
Synchrotron site | APS |
Beamline | 14-BM-C |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2005-10-30 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.979 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 56.397, 81.811, 58.861 |
Unit cell angles | 90.00, 111.16, 90.00 |
Refinement procedure
Resolution | 19.620 - 2.100 |
R-factor | 0.20234 |
Rwork | 0.200 |
R-free | 0.24044 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1zky |
RMSD bond length | 0.008 |
RMSD bond angle | 1.074 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.180 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.102 | 0.421 |
Number of reflections | 29195 | |
<I/σ(I)> | 12.9 | 4.56 |
Completeness [%] | 98.9 | 100 |
Redundancy | 5.1 | 5.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 289 | 0.2M Ammonium Sulfate 0.1M Tris pH 8.5 25% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 289K |