2FA5
The crystal structure of an unliganded multiple antibiotic-resistance repressor (MarR) from Xanthomonas campestris
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL12B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL12B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-06-04 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.979575 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 71.420, 71.420, 110.290 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.780 - 1.800 |
| Rwork | 0.200 |
| R-free | 0.23400 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.100 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.780 | |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 52842 | |
| Completeness [%] | 90.6 | 90.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIQUID DIFFUSION | 5.5 | 293 | 0.1M NaOAC, 0.2M MgCl2, 22% PEG 2K MME, pH 5.5, LIQUID DIFFUSION, temperature 293K |
