2FA1
Crystal structure of PhnF C-terminal domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-10-10 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.97944 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.765, 51.572, 63.961 |
| Unit cell angles | 90.00, 103.64, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.700 |
| R-factor | 0.18958 |
| Rwork | 0.187 |
| R-free | 0.24121 |
| Structure solution method | SAD |
| RMSD bond length | 0.025 |
| RMSD bond angle | 2.332 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 32745 | |
| <I/σ(I)> | 33.1 | 2.75 |
| Completeness [%] | 99.9 | 99.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 297 | 20% PEG3350, 0.2M Mg Formate, 50mMtris pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 297K |
