2F9Y
The Crystal Structure of The Carboxyltransferase Subunit of ACC from Escherichia coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Collection date | 2003-01-17 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 156.729, 156.728, 192.824 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.730 - 3.200 |
| R-factor | 0.257 |
| Rwork | 0.257 |
| R-free | 0.26100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2f9i |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.310 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Number of reflections | 23565 | |
| <I/σ(I)> | 26.6 | 8 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 7.8 | 8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 9.5 | 277 | 1M NaH2PO4, 1M K2HPO4, 2M Li2SO4, 1M CAPS, pH 9.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K, pH 9.50 |
