2F9S

2nd Crystal Structure Of A Soluble Domain Of ResA In The Oxidised Form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2003-11-11
Detector	SBC
Wavelength(s)	0.9641
Spacegroup name	P 65
Unit cell lengths	61.186, 61.186, 166.999
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	52.700 - 1.401
R-factor	0.16273
Rwork	0.162
R-free	0.17529
Structure solution method	MAD
RMSD bond length	0.012
RMSD bond angle	1.747
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	52.700	1.450
High resolution limit [Å]	1.401	1.401
Number of reflections	69296
<I/σ(I)>	17.4	1.5
Completeness [%]	1.0	0.959
Redundancy	5.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.8	293	100mM Tris HCl; 2.2M Ammonium Sulfate; 2mM L-Cysteine, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	6.8	293	100mM Tris HCl; 2.2M Ammonium Sulfate; 2mM L-Cysteine, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K