2F95

M intermediate structure of sensory rhodopsin II/transducer complex in combination with the ground state structure

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2004-09-20
Detector	ADSC QUANTUM 4
Wavelength(s)	0.934
Spacegroup name	P 21 21 2
Unit cell lengths	124.620, 46.900, 53.850
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	22.010 - 2.200
R-factor	0.217
Rwork	0.217
R-free	0.24100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	: 1H2S
RMSD bond length	0.007
RMSD bond angle	1.100
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	22.010	2.260
High resolution limit [Å]	2.200	2.200
Rmerge	0.085	0.414
Number of reflections	16147
Completeness [%]	97.9	99.4
Redundancy	3.9	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		5.8	296	150 MM NACL, 25 MM NAKPI 5.1 0.8% B-OCTYLGLUCOSID , MONOVACCENIN (CUBIC PHASE) PRECIPITATED BY 1 M NA/KPI 5.8 ,in cubic lipidic phase, temperature 296K