2F93

K Intermediate Structure of Sensory Rhodopsin II/Transducer Complex in Combination with the Ground State Structure

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2004-09-20
Detector	ADSC QUANTUM 4
Wavelength(s)	0.934
Spacegroup name	P 21 21 2
Unit cell lengths	124.340, 47.030, 53.820
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.060 - 2.000
R-factor	0.218
Rwork	0.218
R-free	0.24500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	: 1H2S
RMSD bond length	0.009
RMSD bond angle	1.200
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.060	2.050
High resolution limit [Å]	2.000	2.000
Rmerge	0.091	0.494
Number of reflections	21010
Completeness [%]	95.4	98.7
Redundancy	3.8	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		5.8	295	150 MM NACL, 25 MM NAKPI 5.1 0.8% B-OCTYLGLUCOSID , MONOVACCENIN (CUBIC PHASE) PRECIPITATED BY 1 M NA/KPI 5.8 , in cubic lipidic phase, temperature 295K