2F8W
Crystal structure of d(CACGTG)2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | ENRAF-NONIUS |
| Temperature [K] | 291 |
| Detector technology | DIFFRACTOMETER |
| Collection date | 1987-02-04 |
| Detector | ENRAF-NONIUS CAD4 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 18.420, 30.740, 43.180 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 6.000 - 1.200 |
| R-factor | 0.183 |
| Rwork | 0.180 |
| R-free | 0.21500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1d48 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 3.180 |
| Data reduction software | CAD4 (associated program) |
| Data scaling software | CAD4 |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.200 |
| Number of reflections | 5687 |
| Completeness [%] | 70.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 7.5 mM sodium cacodylate, 2.5 mM SrCl2, 2.5 mM spermine, 50% Isopropanol, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | sodium cacodylate | ||
| 2 | 1 | 1 | SrCl2 | ||
| 3 | 1 | 1 | spermine | ||
| 4 | 1 | 1 | Isopropanol | ||
| 5 | 1 | 1 | H2O | ||
| 6 | 1 | 2 | sodium cacodylate | ||
| 7 | 1 | 2 | SrCl2 | ||
| 8 | 1 | 2 | H2O |
