2F8Q
An alkali thermostable F/10 xylanase from alkalophilic Bacillus sp. NG-27
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-03-05 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 174.505, 54.738, 131.497 |
| Unit cell angles | 90.00, 131.21, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.200 |
| R-factor | 0.197 |
| Rwork | 0.197 |
| R-free | 0.23500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hiz |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.200 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 46129 | |
| Completeness [%] | 96.8 | 86 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.5 | 273 | 0.1M NaCl, 0.01M MgCl2, 0.1M Tris pH 8.5 and 18% PEG 8000, VAPOR DIFFUSION, temperature 273K |
