2F8K
Sequence specific recognition of RNA hairpins by the SAM domain of Vts1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-002 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-07-20 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 27.550, 44.530, 129.230 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 64.550 - 2.000 |
| R-factor | 0.23 |
| Rwork | 0.228 |
| R-free | 0.27700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | : 2D3D |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.319 |
| Data reduction software | CrystalClear ((MSC/RIGAKU)) |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 64.550 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 11366 | |
| <I/σ(I)> | 11.37 | 6.43 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 6.4 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 2.5 M Ammonium sulfate, 50 mM MES pH 5.5, 10 mM Magnesium Acetate, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | Ammonium sulfate | ||
| 2 | 1 | 1 | MES | ||
| 3 | 1 | 1 | Magnesium Acetate | ||
| 4 | 1 | 1 | H2O | ||
| 5 | 1 | 2 | Ammonium sulfate | ||
| 6 | 1 | 2 | MES | ||
| 7 | 1 | 2 | Magnesium Acetate |
