2F8J

Crystal structure of Histidinol-phosphate aminotransferase (EC 2.6.1.9) (Imidazole acetol-phosphate transferase) (tm1040) from Thermotoga maritima at 2.40 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2005-02-28
Detector	ADSC QUANTUM 315
Wavelength(s)	1.000001
Spacegroup name	P 21 21 2
Unit cell lengths	145.156, 187.453, 54.316
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.600 - 2.400
R-factor	0.18469
Rwork	0.182
R-free	0.23800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1uu0
RMSD bond length	0.011
RMSD bond angle	1.228
Data reduction software	DENZO
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.800	50.000	2.440
High resolution limit [Å]	2.400	6.510	2.400
Rmerge	0.079	0.055	0.562
Number of reflections	54470
<I/σ(I)>	9.3		1.8
Completeness [%]	91.7	86.7	90.1
Redundancy	2.7	2.6	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP, NANODROP	6.5	293	0.2M MgCl2, 20.0% PEG-1000, 0.1M Cacodylate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, NANODROP, temperature 293K