2F8H
Structure of acetylcitrulline deacetylase from Xanthomonas campestris in metal-free form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X26C |
| Synchrotron site | NSLS |
| Beamline | X26C |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2005-03-30 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 94.133, 95.226, 43.613 |
| Unit cell angles | 90.00, 93.76, 90.00 |
Refinement procedure
| Resolution | 35.720 - 1.750 |
| R-factor | 0.229 |
| Rwork | 0.226 |
| R-free | 0.25800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2f7v |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.720 | 1.810 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.065 | 0.397 |
| Number of reflections | 37941 | |
| <I/σ(I)> | 22.3 | 3.5 |
| Completeness [%] | 98.0 | 96 |
| Redundancy | 7.6 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.9 | 291 | 12-15% (w/v) PEG 3350, 200 mM magnesium formate, pH 5.9, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
