2F8D
BenM effector-Binding domain crystallized from high pH conditions
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-01-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97935 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 94.060, 106.401, 184.306 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 92.060 - 2.700 |
| R-factor | 0.151 |
| Rwork | 0.148 |
| R-free | 0.19600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2f6g |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.083 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 92.060 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.093 | 0.421 |
| Number of reflections | 25480 | |
| <I/σ(I)> | 28.1 | 5.85 |
| Completeness [%] | 98.4 | 98.1 |
| Redundancy | 7.16 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 10 | PEG 4000, glycerol, imidazole, tris, CAPS, KBr, NaCl, benzoate, pH 10, Microbatch under oil |
