2F86

The Association Domain of C. elegans CaMKII

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2004-10-26
Detector	ADSC QUANTUM 4
Wavelength(s)	.9798
Spacegroup name	C 2 2 21
Unit cell lengths	70.870, 186.934, 182.934
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.800 - 2.640
R-factor	0.248
Rwork	0.248
R-free	0.30100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1hkx
RMSD bond length	0.008
RMSD bond angle	1.400
Data reduction software	MADNESS
Data scaling software	SCALEPACK
Phasing software	PHASER (V. 1.3)
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.730
High resolution limit [Å]	2.640	2.640
Number of reflections	33540
Completeness [%]	94.6	85.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.4	293	50 mM HEPES-sodium, pH 6.4, 100 mM KCl, 10 mM MgCl, 5% PEG 400 , VAPOR DIFFUSION, HANGING DROP, temperature 293K