2F84
Crystal Structure of an orotidine-5'-monophosphate decarboxylase homolog from P.falciparum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-09-15 |
Detector | ADSC QUANTUM 4 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 70.911, 69.057, 69.735 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 18.610 - 2.100 |
R-factor | 0.23 |
Rwork | 0.227 |
R-free | 0.28800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2AQW.pdb |
RMSD bond length | 0.016 |
RMSD bond angle | 1.475 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | EPMR (2.5) |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 69.740 | 2.110 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.102 | 0.785 |
Number of reflections | 22698 | |
<I/σ(I)> | 4.9 | 0.7 |
Completeness [%] | 96.1 | 85.8 |
Redundancy | 3.8 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.2 | 298 | 100mM Ammonium Phosphate, 18% PEG 1000, pH 8.2, VAPOR DIFFUSION, SITTING DROP, temperature 298K |