2F7Y

Crystal structure of Molybdenum cofactor biosynthesis protein Mog from Shewanella oneidensis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2005-10-17
Detector	ADSC QUANTUM 4
Wavelength(s)	0.97940
Spacegroup name	P 21 21 2
Unit cell lengths	85.570, 215.359, 40.844
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.300
R-factor	0.19428
Rwork	0.193
R-free	0.22240
Structure solution method	SAD
RMSD bond length	0.023
RMSD bond angle	1.883
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.090	0.364
Number of reflections	34178
<I/σ(I)>	32.32	3.61
Completeness [%]	98.6	93.6
Redundancy	6.2	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	289	2.5 M NaCl 0.1 M Tris pH 7.0 0.2 M MgCl2, VAPOR DIFFUSION, SITTING DROP, temperature 289K