2F7Y
Crystal structure of Molybdenum cofactor biosynthesis protein Mog from Shewanella oneidensis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-10-17 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97940 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 85.570, 215.359, 40.844 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.300 |
| R-factor | 0.19428 |
| Rwork | 0.193 |
| R-free | 0.22240 |
| Structure solution method | SAD |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.883 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.090 | 0.364 |
| Number of reflections | 34178 | |
| <I/σ(I)> | 32.32 | 3.61 |
| Completeness [%] | 98.6 | 93.6 |
| Redundancy | 6.2 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289 | 2.5 M NaCl 0.1 M Tris pH 7.0 0.2 M MgCl2, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
