2F7W
Crystal structure of Molybdenum cofactor biosynthesis protein Mog from Shewanella oneidensis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-06-21 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.97942 |
Spacegroup name | P 32 |
Unit cell lengths | 65.910, 65.910, 101.212 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 1.900 |
R-factor | 0.17979 |
Rwork | 0.177 |
R-free | 0.22845 |
Structure solution method | SAD |
RMSD bond length | 0.015 |
RMSD bond angle | 1.501 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.115 | 0.511 |
Number of reflections | 38213 | |
<I/σ(I)> | 28.9 | 3.94 |
Completeness [%] | 98.6 | 98.3 |
Redundancy | 6.1 | 5.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | 0.2 M Ammonium sulfate 0.1 M HEPES pH 7.5 25 % PEG3350, VAPOR DIFFUSION, SITTING DROP, temperature 289K |