2F7N
Structure of D. radiodurans Dps-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-05-14 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 2 3 |
| Unit cell lengths | 90.130, 90.130, 90.130 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.000 |
| R-factor | 0.2 |
| Rwork | 0.195 |
| R-free | 0.22000 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.100 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.090 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.110 | 0.480 |
| Number of reflections | 16568 | |
| <I/σ(I)> | 14.7 | 1.9 |
| Completeness [%] | 99.8 | 90.3 |
| Redundancy | 9.2 | 8.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 100mM MES; 100mM cobalt chloride; 2M anmmonium sulfate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
