2F7M
Crystal Structure of Unliganded Human FPPS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 96 |
| Detector technology | CCD |
| Collection date | 2004-04-15 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97933 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 110.889, 110.889, 76.996 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.690 - 2.300 |
| R-factor | 0.228 |
| Rwork | 0.225 |
| R-free | 0.25700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fps |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.000 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.700 | 2.370 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.072 | 0.325 |
| Number of reflections | 21795 | 1795 |
| <I/σ(I)> | 23.3 | 8.17 |
| Completeness [%] | 99.4 | 99.9 |
| Redundancy | 14.3 | 14.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.7 | 292 | 1.2M sodium potassium phosphate, 25% glycerol, pH 4.7, VAPOR DIFFUSION, HANGING DROP, temperature 292.0K |
