2F7M
Crystal Structure of Unliganded Human FPPS
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 96 |
Detector technology | CCD |
Collection date | 2004-04-15 |
Detector | MARRESEARCH |
Wavelength(s) | 0.97933 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 110.889, 110.889, 76.996 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.690 - 2.300 |
R-factor | 0.228 |
Rwork | 0.225 |
R-free | 0.25700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1fps |
RMSD bond length | 0.007 |
RMSD bond angle | 1.000 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.700 | 2.370 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.072 | 0.325 |
Number of reflections | 21795 | 1795 |
<I/σ(I)> | 23.3 | 8.17 |
Completeness [%] | 99.4 | 99.9 |
Redundancy | 14.3 | 14.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.7 | 292 | 1.2M sodium potassium phosphate, 25% glycerol, pH 4.7, VAPOR DIFFUSION, HANGING DROP, temperature 292.0K |