2F7L
Crystal structure of Sulfolobus tokodaii phosphomannomutase/phosphoglucomutase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-03-12 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9999 |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 161.904, 161.904, 170.494 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.800 |
| Rwork | 0.225 |
| R-free | 0.28000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1WQA CHAIN A |
| RMSD bond length | 1.627 |
| RMSD bond angle | 0.010 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.082 | 0.573 |
| Number of reflections | 32892 | |
| <I/σ(I)> | 9.4 | 2.19 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 14.5 | 14.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.4 | 293 | 2.0M ammonium sulfate, 0.1M sodium citrate, pH 5.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
