2F74
Murine MHC class I H-2Db in complex with human b2-microglobulin and LCMV-derived immunodminant peptide gp33
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-09-27 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0292 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 68.142, 65.197, 101.941 |
| Unit cell angles | 90.00, 102.43, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.700 |
| R-factor | 0.22672 |
| Rwork | 0.223 |
| R-free | 0.29768 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1s7u |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.042 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.19) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.750 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.049 | 0.315 |
| Number of reflections | 22481 | |
| <I/σ(I)> | 20.2 | 2.4 |
| Completeness [%] | 91.8 | 51.4 |
| Redundancy | 3.7 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8 | 290 | 20% PEG 6K, pH 8, VAPOR DIFFUSION, temperature 290K |
