2F6M
Structure of a Vps23-C:Vps28-N subcomplex
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-06-11 |
Detector | MARRESEARCH |
Wavelength(s) | 0.9190, 0.9800 |
Spacegroup name | I 21 21 21 |
Unit cell lengths | 61.239, 119.252, 125.391 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.510 - 2.100 |
R-factor | 0.24168 |
Rwork | 0.241 |
R-free | 0.26096 |
Structure solution method | MAD |
RMSD bond length | 0.007 |
RMSD bond angle | 0.946 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.170 |
High resolution limit [Å] | 2.100 | 2.100 |
Number of reflections | 25067 | |
<I/σ(I)> | 20.5 | 4.8 |
Completeness [%] | 97.9 | 87.2 |
Redundancy | 6.1 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 277 | 5mg/ml protein solution containing 40 mM TRIS pH 7.4, 120 mM NaCl, and 20 mM DDAO was mixed with equal volume of crystallant containing 13% PEG 3350, 200 mM MgCl2 and 20% glycerol., VAPOR DIFFUSION, HANGING DROP, temperature 277K |
1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 277 | 5mg/ml protein solution containing 40 mM TRIS pH 7.4, 120 mM NaCl, and 20 mM DDAO was mixed with equal volume of crystallant containing 13% PEG 3350, 200 mM MgCl2 and 20% glycerol., VAPOR DIFFUSION, HANGING DROP, temperature 277K |
1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 277 | 5mg/ml protein solution containing 40 mM TRIS pH 7.4, 120 mM NaCl, and 20 mM DDAO was mixed with equal volume of crystallant containing 13% PEG 3350, 200 mM MgCl2 and 20% glycerol., VAPOR DIFFUSION, HANGING DROP, temperature 277K |