2F6H

Myosin V cargo binding domain

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2004-03-27
Detector	ADSC QUANTUM 4
Spacegroup name	P 21 21 21
Unit cell lengths	50.780, 72.673, 167.982
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	12.000 - 2.250
R-factor	0.219
Rwork	0.217
R-free	0.25000
Structure solution method	MAD
RMSD bond length	0.013
RMSD bond angle	1.313
Data reduction software	d*TREK
Data scaling software	d*TREK
Phasing software	SOLVE
Refinement software	REFMAC (5.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.820	2.280
High resolution limit [Å]	2.200	2.200
Rmerge	0.106	0.532
Number of reflections	28080
<I/σ(I)>	8.9	4.1
Completeness [%]	97.0	98.5
Redundancy	5.46	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.5	279	1.7 M Nacl, 6% PEG 8000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 279K, pH 6.50
1		6.5	279	1.7 M Nacl, 6% PEG 8000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 279K, pH 6.50
1		6.5	279	1.7 M Nacl, 6% PEG 8000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 279K, pH 6.50
1		6.5	279	1.7 M Nacl, 6% PEG 8000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 279K, pH 6.50