2F6G

BenM effector binding domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	100
Detector technology	CCD
Detector	MARRESEARCH
Wavelength(s)	0.97937
Spacegroup name	P 21 21 21
Unit cell lengths	64.412, 66.615, 116.549
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	58.321 - 1.908
R-factor	0.19004
Rwork	0.190
R-free	0.23400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2f6p
RMSD bond length	0.007
RMSD bond angle	1.226
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (refmac_5.2.0005)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	58.321	50.000	1.970
High resolution limit [Å]	1.908	4.090	1.900
Rmerge		0.019	0.434
Number of reflections	29271

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	microbatch under oil		288	PEG 4000, ammonium sulfate, sodium acetate, NaCl, tris, glycerol, imidazole, microbatch under oil, temperature 288K