2F6B
Structural and active site modification studies implicate Glu, Trp and Arg in the activity of xylanase from alkalophilic Bacillus sp. (NCL 87-6-10).
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 295 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-12-12 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 73.421, 77.281, 79.233 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 55.050 - 2.800 |
| R-factor | 0.16917 |
| Rwork | 0.166 |
| R-free | 0.22649 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1h4g |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.710 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.071 | 0.071 |
| Number of reflections | 11586 | |
| <I/σ(I)> | 12.64 | 12.64 |
| Completeness [%] | 93.9 | 93.9 |
| Redundancy | 4.127 | 4.127 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | Isopropanal 30%,Sodium citrate 0.2M, Sodium Cacodylate buffer(0.1M) of pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
