2F5Y
Crystal Structure of the PDZ Domain from Human RGS-3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-10-22 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9788 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 91.110, 44.140, 49.857 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.880 - 2.390 |
| R-factor | 0.21698 |
| Rwork | 0.215 |
| R-free | 0.26429 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1I92.pdb 1QAU.pdb |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.484 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.860 | 2.490 |
| High resolution limit [Å] | 2.390 | 2.400 |
| Number of reflections | 8381 | |
| Completeness [%] | 99.7 | 98.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 1.6M ammonium sulphate, 0.1M MES, 10% dioxane, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
