2F5T
Crystal Structure of the sugar binding domain of the archaeal transcriptional regulator TrmB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Wavelength(s) | 0.85, 0.978, 0.979, 1.699 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 56.857, 56.857, 132.482 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.530 - 1.450 |
| Rwork | 0.162 |
| R-free | 0.19500 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.822 |
| Data scaling software | XSCALE |
| Phasing software | SOLVE (2.08) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 39.530 | 39.530 | 1.540 |
| High resolution limit [Å] | 1.450 | 10.000 | 1.450 |
| Rmerge | 0.049 | 0.290 | 0.290 |
| Number of reflections | 44046 | 173 | 6390 |
| <I/σ(I)> | 36.1 | 56.68 | 8.12 |
| Completeness [%] | 97.2 | 93 | 87.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 291 | 100mM sodium acetate, 8%(w/v) PEG 4000, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 291 | 100mM sodium acetate, 8%(w/v) PEG 4000, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 291 | 100mM sodium acetate, 8%(w/v) PEG 4000, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 291 | 100mM sodium acetate, 8%(w/v) PEG 4000, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 291 | 100mM sodium acetate, 8%(w/v) PEG 4000, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 291 | 100mM sodium acetate, 8%(w/v) PEG 4000, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 291 | 100mM sodium acetate, 8%(w/v) PEG 4000, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 291 | 100mM sodium acetate, 8%(w/v) PEG 4000, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 291 | 100mM sodium acetate, 8%(w/v) PEG 4000, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
