2F5K

Crystal structure of the chromo domain of human MRG15

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F2
Synchrotron site	CHESS
Beamline	F2
Temperature [K]	100
Detector technology	CCD
Collection date	2005-07-14
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9793
Spacegroup name	C 1 2 1
Unit cell lengths	109.480, 80.313, 81.285
Unit cell angles	90.00, 123.91, 90.00

Refinement procedure

Resolution	50.000 - 2.200
R-factor	0.22697
Rwork	0.224
R-free	0.27270
Structure solution method	SAD
RMSD bond length	0.014
RMSD bond angle	1.523
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.280
High resolution limit [Å]	2.200	2.200
Number of reflections	29581
Completeness [%]	99.8	99.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.9	277	14% PEG 3350, 0.2M potassium nitrate, pH 6.9, VAPOR DIFFUSION, HANGING DROP, temperature 277K
1	VAPOR DIFFUSION, HANGING DROP	6.9	277	14% PEG 3350, 0.2M potassium nitrate, pH 6.9, VAPOR DIFFUSION, HANGING DROP, temperature 277K