2F5F
Bacillus subtilis manganese transport regulator (MNTR) bound to manganese, AC conformation, pH 8.5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 295 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-06-17 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.630, 46.420, 75.420 |
| Unit cell angles | 90.00, 94.26, 90.00 |
Refinement procedure
| Resolution | 31.700 - 2.400 |
| R-factor | 0.222 |
| Rwork | 0.222 |
| R-free | 0.25700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1on1 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.100 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.700 | 2.550 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.087 | 0.342 |
| Number of reflections | 13217 | |
| <I/σ(I)> | 5.2 | 2 |
| Completeness [%] | 95.0 | 93.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 30% PEG 400, 0.15 M LITHIUM SULFATE, PH 8.5, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K, pH 8.50 |
