2F5D
Bacillus subtilis manganese transport regulator (MNTR) bound to manganese, AC conformation, pH 6.5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU300 |
Temperature [K] | 295 |
Detector technology | IMAGE PLATE |
Collection date | 2003-03-03 |
Detector | RIGAKU RAXIS IV |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 50.730, 46.400, 75.800 |
Unit cell angles | 90.00, 94.61, 90.00 |
Refinement procedure
Resolution | 31.500 - 1.900 |
R-factor | 0.21 |
Rwork | 0.210 |
R-free | 0.23000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1on1 |
RMSD bond length | 0.005 |
RMSD bond angle | 0.900 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 31.500 | 2.020 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.064 | 0.270 |
Number of reflections | 25228 | |
<I/σ(I)> | 6.2 | 2.5 |
Completeness [%] | 90.0 | 61.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | 15% PEG 4000, 0.1 M MAGNESIUM CHLORIDE, 0.1 M CACODYLATE, PH 6.50, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 295K |