2F5D
Bacillus subtilis manganese transport regulator (MNTR) bound to manganese, AC conformation, pH 6.5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 295 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-03-03 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.730, 46.400, 75.800 |
| Unit cell angles | 90.00, 94.61, 90.00 |
Refinement procedure
| Resolution | 31.500 - 1.900 |
| R-factor | 0.21 |
| Rwork | 0.210 |
| R-free | 0.23000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1on1 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.900 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.500 | 2.020 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.064 | 0.270 |
| Number of reflections | 25228 | |
| <I/σ(I)> | 6.2 | 2.5 |
| Completeness [%] | 90.0 | 61.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | 15% PEG 4000, 0.1 M MAGNESIUM CHLORIDE, 0.1 M CACODYLATE, PH 6.50, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 295K |
