2F4Z
Toxoplasma gondii ubiquitin conjugating enzyme TgTwinScan_2721- E2 domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-09-01 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 74.475, 141.334, 77.130 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.110 |
| R-factor | 0.19992 |
| Rwork | 0.199 |
| R-free | 0.23779 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bep |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.892 |
| Data reduction software | CrystalClear ((MSC/RIGAKU)) |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER (V. 1.3.1) |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.160 |
| High resolution limit [Å] | 2.120 | 2.120 |
| Rmerge | 0.077 | 0.729 |
| Number of reflections | 23386 | |
| <I/σ(I)> | 7.6 | 2.22 |
| Completeness [%] | 98.8 | 97.3 |
| Redundancy | 6.3 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.4 | 297 | 32% Peg 1500, 0.2 M MgCl2, 0.1 M Tris pH 8.4, 10 mM Spermidine, VAPOR DIFFUSION, temperature 297K |
