2F4T
Asite RNA + designer antibiotic
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Collection date | 2002-06-01 |
Wavelength(s) | 0.991 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 45.976, 32.926, 46.414 |
Unit cell angles | 90.00, 95.62, 90.00 |
Refinement procedure
Resolution | 15.000 - 3.000 |
R-factor | 0.267 |
Rwork | 0.211 |
R-free | 0.26700 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | CNS |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 3.110 |
High resolution limit [Å] | 3.000 | 6.440 | 3.000 |
Rmerge | 0.078 | 0.040 | 0.206 |
Number of reflections | 2746 | ||
<I/σ(I)> | 8 | 3.2 | |
Completeness [%] | 97.7 | 79.9 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |