2F4Q
Crystal Structure of Deinococcus radiodurans topoisomerase IB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 5ID-B |
| Synchrotron site | APS |
| Beamline | 5ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-07-02 |
| Detector | MARRESEARCH |
| Wavelength(s) | .9768 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 38.094, 64.966, 76.622 |
| Unit cell angles | 90.00, 91.78, 90.00 |
Refinement procedure
| Resolution | 27.500 - 1.750 |
| R-factor | 0.1992 |
| Rwork | 0.197 |
| R-free | 0.23079 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.999 |
| Data scaling software | XDS |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.500 | 1.800 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.051 | 0.227 |
| Number of reflections | 36231 | |
| <I/σ(I)> | 27.5 | 5.7 |
| Completeness [%] | 96.4 | 96.4 |
| Redundancy | 6.3 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 283 | 12% PEG 3350 (w/v), 0.1 M MES (pH 6.5), 0.2 M ammonium chloride, VAPOR DIFFUSION, HANGING DROP, temperature 283K |
