2F4N

Crystal structure of protein MJ1651 from Methanococcus jannaschii DSM 2661, Pfam DUF62

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2005-07-28
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9789
Spacegroup name	C 1 2 1
Unit cell lengths	126.840, 110.080, 75.820
Unit cell angles	90.00, 115.39, 90.00

Refinement procedure

Resolution	39.860 - 2.500
Rwork	0.237
R-free	0.26500
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.300
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.860	2.450
High resolution limit [Å]	2.360	2.360
Rmerge	0.105	0.465
Number of reflections	72852
<I/σ(I)>	11.7
Completeness [%]	99.5
Redundancy	6.6	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	50% MPD, 0.2M Ammonium dihydrogen phosphate, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K