2F4M
The Mouse PNGase-HR23 Complex Reveals a Complete Remodulation of the Protein-Protein Interface Compared to its Yeast Orthologs
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2005-04-23 |
Detector | RIGAKU RAXIS IV |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 98.225, 52.297, 82.548 |
Unit cell angles | 90.00, 115.33, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.850 |
R-factor | 0.189 |
Rwork | 0.186 |
R-free | 0.23900 |
Structure solution method | MIR |
RMSD bond length | 0.016 |
RMSD bond angle | 1.547 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.910 |
High resolution limit [Å] | 1.850 | 1.850 |
Number of reflections | 31216 | |
<I/σ(I)> | 15.5 | 2 |
Completeness [%] | 95.9 | 20.9 |
Redundancy | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.5 | 291 | 0.1 M Tris-HCl, pH 8.5, 28-32% PEG4000, 0.2 M sodium acetate, VAPOR DIFFUSION, HANGING DROP, temperature 291K, pH 8.50 |