2F4F
Crystal structure of IS200 transposase
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 4A |
| Synchrotron site | PAL/PLS |
| Beamline | 4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-04-06 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.07220, 0.97960, 0.97972, 0.97192 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 91.930, 67.924, 65.332 |
| Unit cell angles | 90.00, 128.73, 90.00 |
Refinement procedure
| Resolution | 19.480 - 1.800 |
| R-factor | 0.222 |
| Rwork | 0.222 |
| R-free | 0.23800 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 29289 | |
| Completeness [%] | 97.9 | 85.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 7.5 | 297 | 100mM sodium HEPES (pH 7.5), 25% (w/v) PEG3350, EVAPORATION, temperature 297K |
