2F4A
Triclinic cross-linked lysozyme soaked with thiourea 1.5M
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | LURE BEAMLINE DW32 |
Synchrotron site | LURE |
Beamline | DW32 |
Temperature [K] | 277 |
Detector technology | IMAGE PLATE |
Collection date | 2002-03-13 |
Detector | MARRESEARCH |
Wavelength(s) | 0.964 |
Spacegroup name | P 1 |
Unit cell lengths | 27.280, 32.020, 34.310 |
Unit cell angles | 88.53, 108.51, 111.92 |
Refinement procedure
Resolution | 10.000 - 1.950 |
Rwork | 0.160 |
R-free | 0.16070 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 2f2n |
RMSD bond length | 0.007 |
RMSD bond angle | 0.022 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHELX |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 18.300 | 2.100 |
High resolution limit [Å] | 1.949 | 1.949 |
Rmerge | 0.181 | |
Number of reflections | 6985 | |
<I/σ(I)> | 6.9 | |
Completeness [%] | 95.5 | 94.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 300 | SODIUM NITRATE AS CRYSTALLIZING AGENT CROSS-LINKED BY VAPOR DIFFUSION WITH GLUTARALDEHYDE, PROTIEN CONCENTRATION 20 MG/ML, pH 6.00, VAPOR DIFFUSION, HANGING DROP, temperature 300K |