2F46
Crystal structure of a putative phosphatase (nma1982) from neisseria meningitidis z2491 at 1.41 A resolution
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-10-11 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.019951, 0.979741 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 144.278, 33.466, 59.899 |
| Unit cell angles | 90.00, 96.18, 90.00 |
Refinement procedure
| Resolution | 18.620 - 1.410 |
| R-factor | 0.20104 |
| Rwork | 0.200 |
| R-free | 0.22800 |
| Structure solution method | MAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.452 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 18.620 | 18.620 | 1.450 |
| High resolution limit [Å] | 1.410 | 6.310 | 1.410 |
| Rmerge | 0.041 | 0.030 | 0.276 |
| Number of reflections | 46247 | ||
| <I/σ(I)> | 9 | 15.4 | 2.5 |
| Completeness [%] | 83.4 | 32.3 | |
| Redundancy | 3.1 | 3 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION,SITTING DROP,NANODROP | 8.5 | 277 | 0.2M MgCl2, 30.0% PEG-4000, 0.1M TRIS, pH 8.5, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K |
