2F43
Rat liver F1-ATPase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | SBC-2 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 144.567, 144.567, 362.982 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.970 - 3.000 |
| R-factor | 0.306 |
| Rwork | 0.306 |
| R-free | 0.32000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mab |
| RMSD bond length | 0.018 |
| RMSD bond angle | 2.100 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 73.520 | 3.160 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 29647 | |
| <I/σ(I)> | 12.1 | 3.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 8.7 | 8.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.08 | 296 | 5MM ATP, 5MM MGCL2, 5MM VANADATE, 25MM NAN3, 50MM MOPS, pH 8.08, VAPOR DIFFUSION, SITTING DROP, temperature 296K |
