2F3Z
Calmodulin/IQ-AA domain complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CAMD BEAMLINE GCPCC |
| Synchrotron site | CAMD |
| Beamline | GCPCC |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-04-27 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.24242 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 86.260, 31.370, 64.110 |
| Unit cell angles | 90.00, 115.89, 90.00 |
Refinement procedure
| Resolution | 21.200 - 1.600 |
| R-factor | 0.219 |
| Rwork | 0.219 |
| R-free | 0.27200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2f3y |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.000 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.038 | 0.188 |
| Number of reflections | 19475 | |
| Completeness [%] | 94.2 | 70.1 |
| Redundancy | 3.5 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.3 | 298 | 32% PEG 4000, 50mM TRIS, 50 mM MgCl2, pH 8.3, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
