2F3X
Crystal structure of FapR (in complex with effector)- a global regulator of fatty acid biosynthesis in B. subtilis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-11-10 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9794 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 89.441, 89.441, 162.166 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 63.250 - 3.100 |
R-factor | 0.19 |
Rwork | 0.187 |
R-free | 0.22600 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.020 |
RMSD bond angle | 2.137 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 63.250 | 3.270 |
High resolution limit [Å] | 3.100 | 3.100 |
Rmerge | 0.079 | 0.310 |
Number of reflections | 12596 | |
<I/σ(I)> | 19.3 | 6 |
Completeness [%] | 100.0 | 100 |
Redundancy | 6.9 | 7.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 25% ethylenglycol, 5mM malonyl-CoA, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |