2F3U

Crystal Structure of the glycogen phosphorylase B / N-(beta-D-glucopyranosyl)-N'-cyclopropyl oxalamide complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X13
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X13
Temperature [K]	293
Detector technology	CCD
Collection date	2005-01-21
Detector	ADSC QUANTUM 4
Wavelength(s)	0.8034
Spacegroup name	P 43 21 2
Unit cell lengths	127.957, 127.957, 116.030
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 1.930
R-factor	0.2
Rwork	0.191
R-free	0.21050
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	2prj
RMSD bond length	0.005
RMSD bond angle	1.240
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.960
High resolution limit [Å]	1.930	1.930
Number of reflections	72265
<I/σ(I)>	6.6	1.9
Completeness [%]	99.3	99.1
Redundancy	4	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	SMALL TUBES	6.7	289	10 mM Bes buffer, 3 mM DDT, pH 6.7, SMALL TUBES, temperature 289K