2F3P
Crystal Structure of the glycogen phosphorylase B / N-(beta-D-glucopyranosyl)oxamic acid complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX9.6 |
Synchrotron site | SRS |
Beamline | PX9.6 |
Temperature [K] | 293 |
Detector technology | CCD |
Collection date | 2004-12-15 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.86 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 127.977, 127.977, 115.697 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.940 |
R-factor | 0.193 |
Rwork | 0.193 |
R-free | 0.21640 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 2prj |
RMSD bond length | 0.006 |
RMSD bond angle | 1.264 |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.970 |
High resolution limit [Å] | 1.940 | 1.940 |
Number of reflections | 71101 | |
<I/σ(I)> | 10.7 | 2.2 |
Completeness [%] | 99.6 | 99.5 |
Redundancy | 4.4 | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | SMALL TUBES | 6.7 | 289 | 10 mM Bes buffer, 3 mM DDT, pH 6.7, SMALL TUBES, temperature 289K |