2F3N

Crystal Structure of the native Shank SAM domain.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	110
Detector technology	CCD
Collection date	2002-12-05
Detector	ADSC QUANTUM 315
Wavelength(s)	0.96000
Spacegroup name	P 65
Unit cell lengths	104.715, 104.715, 40.188
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 2.100
R-factor	0.206
Rwork	0.206
R-free	0.25100
Structure solution method	SIR and MAD
RMSD bond length	0.010
RMSD bond angle	1.396
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.200
High resolution limit [Å]	2.100	2.100
Number of reflections	14919
<I/σ(I)>	34.6	9.6
Completeness [%]	99.7	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.2	277	Well solution: 0.1 M HEPES pH 7.2, 0.4M Ammonium Formate, 6.5 mM N-nonyl BD Glucoside. Protein Buffer: 5mM tris pH 8.0, 50 mM NaCl, 20% Glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 277.K