2F3L

Crystal Structure of a Lumenal Rfr-domain protein (Contig83.1_1_243_746) from Cyanothece sp. 51142 at 2.1 Angstrom resolution.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2005-10-18
Detector	ADSC QUANTUM 4
Wavelength(s)	0.979
Spacegroup name	P 32 2 1
Unit cell lengths	61.754, 61.754, 83.708
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 2.110
R-factor	0.2168
Rwork	0.217
R-free	0.25080
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	1.003
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.180
High resolution limit [Å]	2.110	2.110
Rmerge	0.076	0.218
Number of reflections	9861
<I/σ(I)>	25.2	3.56
Completeness [%]	90.0	57.1
Redundancy	6.2	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.1	295	15% PEG 1500, 50 mM sodium chloride, 10 mM Tris, pH 7.1, VAPOR DIFFUSION, HANGING DROP, temperature 295K
1	VAPOR DIFFUSION, HANGING DROP	7.1	295	15% PEG 1500, 50 mM sodium chloride, 10 mM Tris, pH 7.1, VAPOR DIFFUSION, HANGING DROP, temperature 295K