2F3G
IIAGLC CRYSTAL FORM III
Experimental procedure
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 293 |
Detector technology | AREA DETECTOR |
Collection date | 1993-06 |
Detector | XUONG-HAMLIN MULTIWIRE |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 60.040, 70.230, 74.310 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.130 |
R-factor | 0.169 * |
Rwork | 0.169 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1f3g |
RMSD bond length | 0.011 |
RMSD bond angle | 21.000 * |
Data reduction software | SDMS |
Data scaling software | SDMS |
Phasing software | UNPUBLISHED |
Refinement software | TNT (5E) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 20.000 |
High resolution limit [Å] | 2.130 |
Rmerge | 0.036 |
Total number of observations | 44614 * |
Number of reflections | 15616 |
Completeness [%] | 86.0 |
Redundancy | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 8.5 | 30% PEG 4000, 0.2M MGCL2, 0.1M TRIS, PH 8.5 4 DEGREES |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 4 (mg/ml) | |
2 | 1 | reservoir | mPEG4000 | 30 (%) | |
3 | 1 | reservoir | 0.2 (M) | ||
4 | 1 | reservoir | Tris-HCl | 0.1 (M) |