2F3B

Mechanism of displacement of a catalytically essential loop from the active site of fructose-1,6-bisphosphatase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 4
Spacegroup name	I 2 2 2
Unit cell lengths	52.936, 82.497, 164.955
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	5.000 - 1.800
Rwork	0.222
R-free	0.24900
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	SCALE
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	5.000	1.860
High resolution limit [Å]	1.800	1.800
Rmerge		0.095
Number of reflections	32249
Completeness [%]		74.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1